Test Your Knowledge - BIOTECHNOLOGY MCQ PRACTICE
by Saksham Chaudhry : Lancers Convent, Prashant Vihar, New Delhi 110085
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A fresh set of 20 random questions is generated every time you open this page and every time you refresh it.
For each of the following multiple choice questions, choose the most appropriate answer :

1. Disaggregating of cells can be achieved by
A. physical disruption 
B. enzymatic digestion 
C. treating with chelating agents 
D. all of the above 

2. What is meant by 'Organ culture' ?
A. Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid 
B. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal 
C. Cultivation of organs in a laboratory through the synthesis of tissues 
D. The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body 

3. In angiosperm, the endosperm is
A. haploid 
B. diploid 
C. triploid 
D. none of these 

4. During nitrogen fixation the enzyme nitrogenase catalyse the reaction. The reaction is high energy demanding which require approximately
A. 12 ATP 
B. 18 ATP 
C. 25 ATP 
D. 7 ATP 

5. Retroviruses have advantage for being used as vector for animal cells because
A. they cover a wide host range 
B. infection does not lead to cell death, infected cells produce virus over an indefinite period 
C. viral gene expression is driven by strong promoters 
D. all of the above 

6. Which of the following vector can maintain the largest fragment of foreign DNA?
A. YAC 
B. Cosmid 
C. Plasmid 
D. Phage 

7. The event(s) which does not occur during interphase, is/are
A. Chromatin condenses 
B. Protein Synthesis 
C. Organelles replication 
D. DNA replication 

8. Which of the bacteria is considered for biological leaching?
A. T. thiooxidans 
B. T. ferrooxidans 
C. Ferrobacillus terrooxidans 
D. all of these 

9. Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using
A. BACS 
B. expression vectors 
C. YACS 
D. all of these 

10. In the meiotic cell divison in daughter cells are produced by two successive division in which
A. first division is equational, second is reductional 
B. first division is reductional and second is equational 
C. both divisions are reductional 
D. both divisions are equational 

11. A group of about 300 chlorophyll molecule acting together to trap photons and transfer energy to the pigment molecule at the reaction center may be called as
A. harvesting unit 
B. reaction center 
C. photosynthetic unit 
D. chloromass 

12. In monoclonal antibody technology, tumor cells that can replicate endlessly are fused with mammalian cells that produce an antibody. The result of this cell fusion is a
A. hybridoma 
B. myeloma 
C. natural killer cell 
D. lymphoblast 

13. Bacterial cells protect their own DNA from degradation by restriction endonucleases by
A. methylating the DNA at the sites that the enzyme recognizes 
B. deleting all recognition sites from the genome 
C. not producing any restriction endonucleases 
D. having anti restriction endonucleases 

14. Recombinant vaccine for Hepatitis B virus has been synthesized against which of the following antigen?
A. Viral core antigen (HBcAg) 
B. Viral surface antigen (HBsAg) 
C. e antigen (HBeAg) 
D. All of the above 

15. Starch content of potatoes can be increased by using a bacterial gene, known as
A. sucrose phosphate synthase gene 
B. ADP glucose pyrophosphorylase gene 
C. polygalactouranase gene 
D. none of the above 

16. Which of the following is not true about the helper plasmids?
A. These can replicate in Agrobacterium 
B. These help in the mediating conjugation of intermediate vectors 
C. These can't replicate in Agrobacterium 
D. All of the above 

17. Vinegar is typically produced in fed batch reactors because
A. a fed batch reactor can be used to maintain low acetic acid concentrations 
B. a fed batch reactor can be used to maintain low ethanol concentrations 
C. acetic acid bacteria tend to ferment at high ethanol concentrations 
D. all of the above 

18. Bock beer is prepared from
A. roasted germinated barley seeds 
B. rice 
C. wheat 
D. grapes 

19. Transmission of a gene from male parent to female child to male grand child is known as
A. holandric inheritance 
B. quantitative inheritance 
C. criss-cross inheritance 
D. maternal inheritance 

20. Toxicity due to accumulation of ammonia can be overcome
A. by substituting glutamine by glutamate 
B. by controlled addition of glutamine at low level 
C. by removal of ammonia or ammonium from culture medium 
D. all of the above 

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