Test Your Knowledge - Free MCQ PRACTICE
BIOCHEMISTRY
by Dr K Chaudhry
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A fresh set of 20 random questions is generated every time you open this page and every time you refresh it.
For each of the following multiple choice questions, choose the most appropriate answer :

1. Real time PCR true is used for?
A. DNA Detection only
B. RNA Detection only
C. Protein Detection only
D. DNA Detection and ampliication

2. Guthrie’s bacterial inhibition test for PKU detects
A. Phenylalanine
B. Phenylpyruvate
C. Phenyl lactate
D. All of these

3. In active transport, the membrane structure that functions is
A. cholesterol
B. integral proteins
C. carbohydrates
D. hydrophobic molecules

4. Beta pleated sheets are examples of protein's
A. primary structure
B. secondary structure
C. tertiary structure
D. quaternary structure

5. In gas chromatography, the basis for separation of the components of the volatile material is the difference in
A. partition coefficients
B. conductivity
C. molecular weight
D. molarity

6. Better understanding of the nuclei is possible,
A. with the help of wavelength spectrum
B. with the help of frequencies ranges
C. with the help of a mathematical translator called the fourier transfer algorithm
D. none of the above

7. Which of the following mineral deficiency may result into impaired growth and development, skin lesions and loss of appetite?
A. Zinc
B. Cobalt
C. Iron
D. Magnesium

8. For specific antigen recognition by T cells,
A. antigen is bound by a T cell membrane antibody
B. denaturation of antigen does not reduce epitope recognition
C. MHC molecules are not required
D. antigen exposure during T cell maturation is required

9. Carbon skeleton of all of the following amino acids enter the TCA cycle at the level of ?-keto glutarate, EXCEPT
A. Proline
B. Methionine
C. Arginine
D. Histidine

10. Which of the following is not a necessary component of translation?
A. Anticodon
B. mRNA
C. Ligase
D. Amino acid

11. Why does the glycolytic pathway continue in the direction of glucose catabolism?
A. There are essentially three irreversible reactions that act as the driving force for the pathway
B. High levels of ATP keep the pathway going in a forward direction
C. The enzymes of glycolysis only function in one direction
D. Glycolysis occurs in either direction

12. For gene transfer to be effective, transforming DNA must be
A. incorporated into the bacterial chromosome
B. incorporated into a viral genome
C. free in the bacterial cytoplasm
D. none of the above

13. Where in a eukaryotic cell, DNA can be found?
A. Nucleus
B. Mitochondrion
C. Vacuole
D. Both (a) and (b)

14. Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
A. positive direction
B. 3' to 5' direction
C. 5' to 3' direction
D. negative direction

15. The complex of RNA polymerase, DNA template and new RNA transcript is called
A. transcription bubble
B. replication bubble
C. a translation bubble
D. none of these

16. Ascorbic acid acts as an
A. reducing agent
B. oxidizing agent
C. oxidizing and reducing agent both
D. none of the above

17. Ammonia is detoxified in brain into
A. Urea
B. Glutamine
C. GABA
D. Glutamate

18. Which is the only amino acid that undergoes significant amount of oxidative deamination in the hepatic mitochondira?
A. Lysine
B. Alanine
C. Threonine
D. Glutamate

19. Promoters for tRNAs are located
A. upstream from the start codon
B. downstream from the start codon
C. both (a) and (b)
D. none of these

20. Which of the following has been used as an evidence that primitive life forms lacked both DNA and enzymes?
A. RNA can both code genetic information and act as a catalyst
B. DNA and enzymes are only present in the most advanced cells
C. Advanced cells lack RNA
D. All of the above

Dr K Chaudhry 5 Alpha Inmternational City, Fatehabad 125050 M: 9810570740, 9810571993facebook.com/doctorkc