Test Your Knowledge - Free MCQ PRACTICE
BIOTECHNOLOGY
by Dr K Chaudhry
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A fresh set of 20 random questions is generated every time you open this page and every time you refresh it.
For each of the following multiple choice questions, choose the most appropriate answer :

1. Retroviruses have advantage for being used as vector for animal cells because
A. they cover a wide host range
B. infection does not lead to cell death, infected cells produce virus over an indefinite period
C. viral gene expression is driven by strong promoters
D. all of the above

2. If starch containing substrates are used for ethanol production, yeast strain can't be used directly because
A. it doesn't contain amylases to hydrolyze starch
B. starch is not a suitable substrate for the production of ethanol
C. it is converted to pentose sugar
D. none of the above

3. If the RBC of human were kept in 0.9% NaCl solution, what sort of change will occur to RBC?
A. Cell will Burst
B. Cell will shrink
C. No change to RBC
D. RBC will destroy

4. Which of the following is not true about synthetic seeds?
A. Can be stored for a year without the loss of valuables
B. Easy to handle
C. Can be directly sown in the soil like natural seeds
D. Need hardening in the green house

5. A group of about 300 chlorophyll molecule acting together to trap photons and transfer energy to the pigment molecule at the reaction center may be called as
A. harvesting unit
B. reaction center
C. photosynthetic unit
D. chloromass

6. Which of the following has been confirmed on sugarcane genotypes?
A. Bacilliform virus
B. Cytomegalovirus
C. Varicella-zoster virus
D. Erythrovirus

7. Which of the following is true for the ale beer?
A. Top yeast of S cerevisiae is used
B. Fermentation is quick and completes in 5-7 days
C. Quantity of hops used is more than in lager beer
D. All of the above

8. The yeast generated during the fermentation of beer is generally separated by
A. centrifugation
B. filtration
C. cell disruption
D. all of these

9. Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
A. DNA polymerase
B. Restriction endonuclease
C. DNA ligase
D. DNA helicase

10. Maximum size of foreign DNA that can be inserted into a replacement vector is
A. 25-30 kb
B. 18-20 kb
C. 20-25 kb
D. 40-50 kb

11. The tumor phenotype, which can be maintained indefinitely in tissue culture, results from the expression of genes on the
A. T-DNA
B. c-DNA
C. r-DNA
D. m-RNA

12. Recombinant adeno virus can be created by deleting E1A/E 1B genes that are
A. transcriptional regulators and are essential for viral replication
B. not necessary for viral replication
C. coding for virus coat protein
D. important for packaging of viral DNA into viral head

13. Fermentation medium for oxytetracyclin (terramycin) consist of
A. CSL, starch, (NH4)2 SO4, sodium chloride and CaCO3
B. CSL, (NH4)2 SO4, sodium chloride and CaCO3
C. CSL, starch,(NH4)2 SO4, ammonium chloride and CaCO3
D. CSL,(NH4)2 SO4, ammonium chloride and CaCO3

14. Virulence trait of Agrobacterium tumefaciens is borne on
A. chromosomal DNA
B. tumour inducing plasmid DNA
C. both chromosomal and plasmid DNA
D. cryptic plasmid DNA

15. Difference between ? gt 10 and ? gt 11 vectors is that
A. ? gt 11 is an expression vector
B. ? gt 10 is an expression vector
C. ? gt 10 is a replacement vector
D. ? gt 11 is a replacement vector

16. The Southern blotting technique depends on
A. similarities between the sequences of probe DNA and experimental DNA
B. similarities between the sequences of probe RNA and experimental RNA
C. similarities between the sequences of probe protein and experimental protein
D. the molecular mass of proteins

17. Any system that is not at equilibrium
A. is thermodynamically unstable, although it may be kinetically stable
B. is kinetically unstable, although it may be thermodynamically stable
C. is rushing toward equilibrium at a very rapid rate
D. requires an enzyme to reach equilibrium

18. Agrobacterium tumefaciens is
A. a disease in humans that causes loss of sight
B. a bacterium that can be used to introduce DNA into plants
C. a fungi that is used to produce antibiotics in large amounts
D. a disease in humans that causes loss of weight

19. The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as
A. laser
B. DNA probe
C. gene gun
D. inoculating needle

20. What is the desirable sugar content of the grapes required for the wine production?
A. 2-5%
B. 5-10%
C. 10-14%
D. 14-20%

Dr K Chaudhry 5 Alpha Inmternational City, Fatehabad 125050 M: 9810570740, 9810571993facebook.com/doctorkc