Test Your Knowledge - Free MCQ PRACTICE
BIOTECHNOLOGY
by Dr K Chaudhry
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A fresh set of 20 random questions is generated every time you open this page and every time you refresh it.
For each of the following multiple choice questions, choose the most appropriate answer :

1. Recombinant vaccine for Hepatitis B virus has been synthesized against which of the following antigen?
A. Viral core antigen (HBcAg)
B. Viral surface antigen (HBsAg)
C. e antigen (HBeAg)
D. All of the above

2. The cross linkage of antigens by antibodies is known as
A. agglutination
B. complement fixation
C. a cross reaction
D. all of these

3. Which of the following from a blood clot stimulates the growth of cells in the healing process?
A. EGF
B. PDGF
C. MPF
D. NGF

4. Which of the following functional groups would make a carbon-based compound the least polar?
A. Phosphate
B. Methyl
C. Carboxyl
D. Amino

5. What is the approximate size (in kb) of the melon mitochondrial genome?
A. 600 kilobase
B. 1200 kilobase
C. 2400 kilobase
D. 3000 kilobase

6. In angiosperm, the endosperm is
A. haploid
B. diploid
C. triploid
D. none of these

7. Retroviruses have advantage for being used as vector for animal cells because
A. they cover a wide host range
B. infection does not lead to cell death, infected cells produce virus over an indefinite period
C. viral gene expression is driven by strong promoters
D. all of the above

8. The Southern blotting technique depends on
A. similarities between the sequences of probe DNA and experimental DNA
B. similarities between the sequences of probe RNA and experimental RNA
C. similarities between the sequences of probe protein and experimental protein
D. the molecular mass of proteins

9. Bacterial cells protect their own DNA from degradation by restriction endonucleases by
A. methylating the DNA at the sites that the enzyme recognizes
B. deleting all recognition sites from the genome
C. not producing any restriction endonucleases
D. having anti restriction endonucleases

10. What type of long chain alcohol is esterified to plant chlorophyll molecules?
A. Dolichol
B. Farnesol
C. Phytol
D. None of these

11. When an organism has a life cycle with alternation of generations, the haploid generation is the
A. zygote
B. gametophyte
C. gamete
D. sporophyte

12. Which tropical fruit crop has been successfully engineered to be protected against a lethal virus?
A. Passion fruit
B. Papaya
C. Mango
D. Lychee

13. Which of the following bacteria genus is capable of oxidizing ammonia (NH4)?
A. Nitrospina
B. Nitrobacter
C. Nitrosococcus
D. Nitrosobacter

14. Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA
A. episomally only
B. integrated only
C. either episomally or integrated at high copy number
D. either episomally or integrated at low copy number

15. Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as
A. photoreactivation
B. gel electrophoresis
C. autoradiography
D. blotting

16. A genomic library is
A. a database where the sequence of an organism's genome is stored
B. a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
C. a book that describes how to isolate DNA from a particular organism
D. a place where the information of the genetic organization of organisms are kept.

17. What is the normal role of restriction endonucleases in bacterial cells?
A. To degrade the bacterial chromosome into small pieces during replication
B. To degrade invading phage DNA
C. To produce RNA primers for replication
D. All of the above

18. Biochips are made up of
A. semi-conducting molecules inserted into the protein frame work
B. conducting molecules inserted into the protein frame work
C. non-conducting molecules inserted into the protein frame work
D. any of the above

19. Final alcohol content in wine varies from
A. 6-9 % by weight
B. 8-13 % by weight
C. both A and B
D. 13-15 % by weight

20. Starch content of potatoes can be increased by using a bacterial gene, known as
A. sucrose phosphate synthase gene
B. ADP glucose pyrophosphorylase gene
C. polygalactouranase gene
D. none of the above

Dr K Chaudhry 5 Alpha Inmternational City, Fatehabad 125050 M: 9810570740, 9810571993facebook.com/doctorkc